NovaFluor Blue 760
NF-B760
NovaFluor Blue 760 dye has a unique emission spectrum when excited by the 488 nm laser, which fits in a spectral space that is currently not accessible by other spectral dyes. Adding spectrally unique dyes may allow for building out larger panels with minimal loss of resolution. NovaFluor Blue 760 dye should generally be paired with moderately to highly expressed antigens in multicolor panels. The macromolecule-based NovaFluor Blue 760 dye produces highly stable fluorescence, and stained samples retain their fluorescence intensity and spectral signature when stored at 4°C. Benefits of NovaFluor Blue 760 dye include: 1) Narrow emission spectrum and minimal cross-laser excitation for better resolution 2) Should be paired with moderately expressed antigens to minimize spillover 3) Spectrally unique and can be used to fill unused detectors in conventional flow cytometry 4) Produces stable fluorescence and is compatible with all buffers tested
Excitation & emission spectra
Key facts
- Excitation maximum
- 490 nm
- Emission maximum
- 764 nm
- Optimal laser line
- 488
- Optimal filter
- 780/60
- Relative brightness
- 3 of 5
- Commercial antibodies
- 54 from 1 supplier
NovaFluor Blue 760 has an excitation maximum at 490 nm and an emission maximum at 764 nm, a Stokes shift of 274 nm. It is optimally excited by the 488 laser line.
Frequently asked questions
- What is NovaFluor Blue 760?
- NovaFluor Blue 760 dye has a unique emission spectrum when excited by the 488 nm laser, which fits in a spectral space that is currently not accessible by other spectral dyes. Adding spectrally unique dyes may allow for building out larger panels with minimal loss of resolution. NovaFluor Blue 760 dye should generally be paired with moderately to highly expressed antigens in multicolor panels. The macromolecule-based NovaFluor Blue 760 dye produces highly stable fluorescence, and stained samples retain their fluorescence intensity and spectral signature when stored at 4°C. Benefits of NovaFluor Blue 760 dye include: 1) Narrow emission spectrum and minimal cross-laser excitation for better resolution 2) Should be paired with moderately expressed antigens to minimize spillover 3) Spectrally unique and can be used to fill unused detectors in conventional flow cytometry 4) Produces stable fluorescence and is compatible with all buffers tested
- What laser excites NovaFluor Blue 760?
- NovaFluor Blue 760 is optimally excited by the 488 laser line. Its excitation maximum is 490 nm.
- What filter should I use to detect NovaFluor Blue 760?
- A 780/60 filter captures the peak of the NovaFluor Blue 760 emission near 764 nm with minimal spillover.
- How bright is NovaFluor Blue 760?
- NovaFluor Blue 760 has a relative brightness of 3 out of 5.
- How many antibodies are available conjugated to NovaFluor Blue 760?
- FluoroFinder tracks 54 commercially available NovaFluor Blue 760 conjugates, searchable by target, clone, host and application.