RealYellow 610
RY610
RealYellow™610 from BD Biosciences is a spectral equivalent to BD Horizon™ PE-CF594 (BD Biosciences) and PE-Dazzle™ 594 (BioLegend) however with more discrete excitation by the 561nm laser and minimal cross-laser excitation off the 488-nm blue laser. This limited cross-beam excitation will translate to lower complexity index scores and lower spillover spreading error in high parameter spectral flow cytometry applications than PE tandem fluorophores.
Excitation & emission spectra
Key facts
- Excitation maximum
- 557 nm
- Emission maximum
- 610 nm
- Optimal laser line
- 561
- Relative brightness
- 4 of 5
- Commercial antibodies
- 810 from 1 supplier
RealYellow 610 has an excitation maximum at 557 nm and an emission maximum at 610 nm, a Stokes shift of 53 nm. It is optimally excited by the 561 laser line.
Frequently asked questions
- What is RealYellow 610?
- RealYellow™610 from BD Biosciences is a spectral equivalent to BD Horizon™ PE-CF594 (BD Biosciences) and PE-Dazzle™ 594 (BioLegend) however with more discrete excitation by the 561nm laser and minimal cross-laser excitation off the 488-nm blue laser. This limited cross-beam excitation will translate to lower complexity index scores and lower spillover spreading error in high parameter spectral flow cytometry applications than PE tandem fluorophores.
- What laser excites RealYellow 610?
- RealYellow 610 is optimally excited by the 561 laser line. Its excitation maximum is 557 nm.
- How bright is RealYellow 610?
- RealYellow 610 has a relative brightness of 4 out of 5.
- How many antibodies are available conjugated to RealYellow 610?
- FluoroFinder tracks 810 commercially available RealYellow 610 conjugates, searchable by target, clone, host and application.