RealBlue 744
RB744
RealBlue™ 744 from BD Biosciences is a novel multimer fluorophore for use in spectral flow cytometry applications with an optimal excitation peak at 488nm and an emission peak at 744nm. The emission peak fits neatly between the PerCP-Cy5.5/ StarBright Blue 700/ RealBlue™ 705 and the RealBlue™ 780 emission peaks which means it does not fall into a standard filter set on conventional, non-spectral flow cytometers. It exhibits minimal cross-beam excitation off the 355nm UV, 405nm violet, 561nm yellow/green and 633/647nm red lasers resulting in low potential for spillover spreading error in a multicolor flow cytometry assay.
Excitation & emission spectra
Key facts
- Excitation maximum
- 488 nm
- Emission maximum
- 744 nm
- Optimal laser line
- 488
- Relative brightness
- 5 of 5
- Commercial antibodies
- 804 from 1 supplier
RealBlue 744 has an excitation maximum at 488 nm and an emission maximum at 744 nm, a Stokes shift of 256 nm. It is optimally excited by the 488 laser line.
Frequently asked questions
- What is RealBlue 744?
- RealBlue™ 744 from BD Biosciences is a novel multimer fluorophore for use in spectral flow cytometry applications with an optimal excitation peak at 488nm and an emission peak at 744nm. The emission peak fits neatly between the PerCP-Cy5.5/ StarBright Blue 700/ RealBlue™ 705 and the RealBlue™ 780 emission peaks which means it does not fall into a standard filter set on conventional, non-spectral flow cytometers. It exhibits minimal cross-beam excitation off the 355nm UV, 405nm violet, 561nm yellow/green and 633/647nm red lasers resulting in low potential for spillover spreading error in a multicolor flow cytometry assay.
- What laser excites RealBlue 744?
- RealBlue 744 is optimally excited by the 488 laser line. Its excitation maximum is 488 nm.
- How bright is RealBlue 744?
- RealBlue 744 has a relative brightness of 5 out of 5.
- How many antibodies are available conjugated to RealBlue 744?
- FluoroFinder tracks 804 commercially available RealBlue 744 conjugates, searchable by target, clone, host and application.