STAR 520L
STAR 520L (Ex-Max 521 nm/Em-Max 633 nm)
abberior STAR 520L is a long Stokes-shift dye optimized for STED and confocal microscopy in the orange-red spectral range. It shows a Stokes-shift of approx. 90 nm. As all long Stokes-shift dyes, it shows broad excitation and emission band: It can be excited between 490 - 550 nm and emits between 600 - 720 nm. For STED microscopy, abberior STAR 520L can be most efficiently used with STED laser wavelengths between 750 - 800 nm. abberior STAR 520L is designed and tested for multicolor STED microscopy in combination with abberior STAR 580 and abberior STAR RED using a single STED wavelength (775 nm). The hydrophilic properties of abberior STAR 520L conjugates reduce unspecific binding of this dye and thus decrease undesired background fluorescence. Our abberior STAR 520L can substitute dyes like DY-520XL® or DY-521XL®. abberior STAR 520SXP has been renamed abberior STAR 520L. The structure of the dye has not changed. Best results are obtained with freshly prepared samples.
Excitation & emission spectra
Key facts
- Excitation maximum
- 521 nm
- Emission maximum
- 633 nm
- Optimal laser line
- 488
- Extinction coefficient
- 60000
STAR 520L has an excitation maximum at 521 nm and an emission maximum at 633 nm, a Stokes shift of 112 nm. Its extinction coefficient is 60000 M⁻¹cm⁻¹. It is optimally excited by the 488 laser line.
Frequently asked questions
- What is STAR 520L?
- abberior STAR 520L is a long Stokes-shift dye optimized for STED and confocal microscopy in the orange-red spectral range. It shows a Stokes-shift of approx. 90 nm. As all long Stokes-shift dyes, it shows broad excitation and emission band: It can be excited between 490 - 550 nm and emits between 600 - 720 nm. For STED microscopy, abberior STAR 520L can be most efficiently used with STED laser wavelengths between 750 - 800 nm. abberior STAR 520L is designed and tested for multicolor STED microscopy in combination with abberior STAR 580 and abberior STAR RED using a single STED wavelength (775 nm). The hydrophilic properties of abberior STAR 520L conjugates reduce unspecific binding of this dye and thus decrease undesired background fluorescence. Our abberior STAR 520L can substitute dyes like DY-520XL® or DY-521XL®. abberior STAR 520SXP has been renamed abberior STAR 520L. The structure of the dye has not changed. Best results are obtained with freshly prepared samples.
- What laser excites STAR 520L?
- STAR 520L is optimally excited by the 488 laser line. Its excitation maximum is 521 nm.