LIVE 550
LIVE 550 (Ex-Max 551 nm/Em-Max 573 nm)
abberior LIVE 550 is a member of a new class of fluorescent dyes for live-cell imaging exclusively offered by abberior. This dye is highly photostable and bright orange fluorescent dye, which can be effectively excited with a 561 nm laser. For STED, a depletion wavelength between 650 - 775 nm is recommended. Due to the unique isomeric structure the favorited conformation is stabilized, leading to an enhanced cell permeability of the dye. abberior LIVE 550 can therefore be used in very low nanomolar concentration, reducing background and cytotoxicity effectively, allowing long-term STED and confocal imaging of living cells or tissue. Our abberior LIVE 550 is the ideal partner for abberior LIVE 610 to obtain optimal 2 color live-cell STED and confocal results.
Excitation & emission spectra
Key facts
- Excitation maximum
- 551 nm
- Emission maximum
- 573 nm
- Optimal laser line
- 561
- Extinction coefficient
- 75000
LIVE 550 has an excitation maximum at 551 nm and an emission maximum at 573 nm, a Stokes shift of 22 nm. Its extinction coefficient is 75000 M⁻¹cm⁻¹. It is optimally excited by the 561 laser line.
Frequently asked questions
- What is LIVE 550?
- abberior LIVE 550 is a member of a new class of fluorescent dyes for live-cell imaging exclusively offered by abberior. This dye is highly photostable and bright orange fluorescent dye, which can be effectively excited with a 561 nm laser. For STED, a depletion wavelength between 650 - 775 nm is recommended. Due to the unique isomeric structure the favorited conformation is stabilized, leading to an enhanced cell permeability of the dye. abberior LIVE 550 can therefore be used in very low nanomolar concentration, reducing background and cytotoxicity effectively, allowing long-term STED and confocal imaging of living cells or tissue. Our abberior LIVE 550 is the ideal partner for abberior LIVE 610 to obtain optimal 2 color live-cell STED and confocal results.
- What laser excites LIVE 550?
- LIVE 550 is optimally excited by the 561 laser line. Its excitation maximum is 551 nm.