PerCP-Fire 806
PerCP-Fire 806 (Ex-Max 481 nm/Em-Max 802 nm)
PerCP/Fire™ 806 is an innovative and unique flow cytometry fluorophore excited by the 488 nm blue laser that emits beyond 800 nm. It can be used on conventional cytometers with a 780/60 filter, but is optimal for spectral instruments with both blue and yellow/green lasers. This allows it to be unmixed from dyes like PE/Cyanine7 and PE/Fire™ 810. Its potent brightness makes it suitable for antigens with low expression levels.
Excitation & emission spectra
Key facts
- Excitation maximum
- 483 nm
- Emission maximum
- 802 nm
- Optimal laser line
- 488
- Optimal filter
- 780/60
- Relative brightness
- 3 of 5
- Commercial antibodies
- 350 from 2 suppliers
PerCP-Fire 806 has an excitation maximum at 483 nm and an emission maximum at 802 nm, a Stokes shift of 319 nm. It is optimally excited by the 488 laser line.
Frequently asked questions
- What is PerCP-Fire 806?
- PerCP/Fire™ 806 is an innovative and unique flow cytometry fluorophore excited by the 488 nm blue laser that emits beyond 800 nm. It can be used on conventional cytometers with a 780/60 filter, but is optimal for spectral instruments with both blue and yellow/green lasers. This allows it to be unmixed from dyes like PE/Cyanine7 and PE/Fire™ 810. Its potent brightness makes it suitable for antigens with low expression levels.
- What laser excites PerCP-Fire 806?
- PerCP-Fire 806 is optimally excited by the 488 laser line. Its excitation maximum is 483 nm.
- What filter should I use to detect PerCP-Fire 806?
- A 780/60 filter captures the peak of the PerCP-Fire 806 emission near 802 nm with minimal spillover.
- How bright is PerCP-Fire 806?
- PerCP-Fire 806 has a relative brightness of 3 out of 5.
- How many antibodies are available conjugated to PerCP-Fire 806?
- FluoroFinder tracks 350 commercially available PerCP-Fire 806 conjugates, searchable by target, clone, host and application.