RealBlue 545
RealBlue 545 (Ex-Max 496 nm/Em-Max 545 nm)
BD Horizon RealBlue™ 545 (RB545) is part of BD's new product line of laser-specific fluorochromes which are designed to minimize cross laser excitaiton. This fluorochrome has a maximum excitation wavelength of 496nm and a maximum emission wavelength of 545nm. While RB545 is excited by the 488nm blue laser, it offers miminal cross-laser excitation off of the 561nm yellow/green laser. It is designed for use in Flow Cytometry and Spectral Flow Cytometry.
Excitation & emission spectra
Key facts
- Excitation maximum
- 496 nm
- Emission maximum
- 545 nm
- Optimal laser line
- 488
- Relative brightness
- 2 of 5
- Commercial antibodies
- 456 from 1 supplier
RealBlue 545 has an excitation maximum at 496 nm and an emission maximum at 545 nm, a Stokes shift of 49 nm. It is optimally excited by the 488 laser line.
Frequently asked questions
- What is RealBlue 545?
- BD Horizon RealBlue™ 545 (RB545) is part of BD's new product line of laser-specific fluorochromes which are designed to minimize cross laser excitaiton. This fluorochrome has a maximum excitation wavelength of 496nm and a maximum emission wavelength of 545nm. While RB545 is excited by the 488nm blue laser, it offers miminal cross-laser excitation off of the 561nm yellow/green laser. It is designed for use in Flow Cytometry and Spectral Flow Cytometry.
- What laser excites RealBlue 545?
- RealBlue 545 is optimally excited by the 488 laser line. Its excitation maximum is 496 nm.
- How bright is RealBlue 545?
- RealBlue 545 has a relative brightness of 2 out of 5.
- How many antibodies are available conjugated to RealBlue 545?
- FluoroFinder tracks 456 commercially available RealBlue 545 conjugates, searchable by target, clone, host and application.