RealBlue 780
RealBlue 780 (Ex-Max 488 nm/Em-Max 780 nm)
BD Horizon RealBlue™ 780 (RB780) is part of BD's new product line of laser-specific fluorochromes which are designed to minimize cross laser excitaiton. This fluorochrome has a maximum excitation wavelength of 488nm and a maximum emission wavelength of 780nm. While RB780 is excited by the 488nm blue laser, it offers miminal cross-laser excitation off of the 561nm yellow/green laser. It is designed for use in Flow Cytometry and Spectral Flow Cytometry.
Excitation & emission spectra
Key facts
- Excitation maximum
- 488 nm
- Emission maximum
- 780 nm
- Optimal laser line
- 488
- Optimal filter
- 780/60
- Relative brightness
- 5 of 5
- Commercial antibodies
- 927 from 1 supplier
RealBlue 780 has an excitation maximum at 488 nm and an emission maximum at 780 nm, a Stokes shift of 292 nm. It is optimally excited by the 488 laser line.
Frequently asked questions
- What is RealBlue 780?
- BD Horizon RealBlue™ 780 (RB780) is part of BD's new product line of laser-specific fluorochromes which are designed to minimize cross laser excitaiton. This fluorochrome has a maximum excitation wavelength of 488nm and a maximum emission wavelength of 780nm. While RB780 is excited by the 488nm blue laser, it offers miminal cross-laser excitation off of the 561nm yellow/green laser. It is designed for use in Flow Cytometry and Spectral Flow Cytometry.
- What laser excites RealBlue 780?
- RealBlue 780 is optimally excited by the 488 laser line. Its excitation maximum is 488 nm.
- What filter should I use to detect RealBlue 780?
- A 780/60 filter captures the peak of the RealBlue 780 emission near 780 nm with minimal spillover.
- How bright is RealBlue 780?
- RealBlue 780 has a relative brightness of 5 out of 5.
- How many antibodies are available conjugated to RealBlue 780?
- FluoroFinder tracks 927 commercially available RealBlue 780 conjugates, searchable by target, clone, host and application.