What is Spark Violet 500?

Spark Violet 500 is a green emitting fluorochrome excited by the 405 nm violet laser. This dye is part of the Spark dye family from BioLegend, which consists of many synthetic fluorochrome options known for their stability, solubility, narrow emission bands, and are ideal for use in either conventional or spectral flow cytometry.

What laser excites Spark Violet 500?

Spark Violet 500 is optimally excited by the 405 laser line. Its excitation maximum is 393 nm.

What filter should I use to detect Spark Violet 500?

A 510/50 filter captures the peak of the Spark Violet 500 emission near 500 nm with minimal spillover.

How bright is Spark Violet 500?

Spark Violet 500 has a relative brightness of 1 out of 5.

How many antibodies are available conjugated to Spark Violet 500?

FluoroFinder tracks 67 commercially available Spark Violet 500 conjugates, searchable by target, clone, host and application.

Spark Violet 500 Dye Profile

Excitation & emission spectra

Key facts

Excitation maximum: 393 nm
Emission maximum: 500 nm
Optimal laser line: 405
Optimal filter: 510/50
Relative brightness: 1 of 5
Commercial antibodies: 67 from 2 suppliers

Spark Violet 500 has an excitation maximum at 393 nm and an emission maximum at 500 nm, a Stokes shift of 107 nm. It is optimally excited by the 405 laser line.

Frequently asked questions

What is Spark Violet 500?: Spark Violet 500 is a green emitting fluorochrome excited by the 405 nm violet laser. This dye is part of the Spark dye family from BioLegend, which consists of many synthetic fluorochrome options known for their stability, solubility, narrow emission bands, and are ideal for use in either conventional or spectral flow cytometry.
What laser excites Spark Violet 500?: Spark Violet 500 is optimally excited by the 405 laser line. Its excitation maximum is 393 nm.
What filter should I use to detect Spark Violet 500?: A 510/50 filter captures the peak of the Spark Violet 500 emission near 500 nm with minimal spillover.
How bright is Spark Violet 500?: Spark Violet 500 has a relative brightness of 1 out of 5.
How many antibodies are available conjugated to Spark Violet 500?: FluoroFinder tracks 67 commercially available Spark Violet 500 conjugates, searchable by target, clone, host and application.