StarBright Blue 675
StarBright Blue 675 (Ex-Max 476 nm/Em-Max 675 nm)
StarBright Blue 675 (SBB675) Dye is a very bright, 488 nm laser excitable dye with a unique narrow excitation and emission profile and is suitable for conventional and full spectrum flow cytometry. It is brighter than other 488 nm laser excitable dyes such as PerCP-Cy5.5 (and PerCP) and BB700 and PE-Cy5 when excited by the 488 nm laser. As it is not a traditional tandem dye, SBB675 does not have issues around tandem breakdown and changes to staining over time. It exhibits reduced excitation by the 561 nm laser compared to PE tandem dyes. SBB675 provides more choice and flexibility when building multicolor panels incorporating the 488 nm laser.
Excitation & emission spectra
Key facts
- Excitation maximum
- 476 nm
- Emission maximum
- 675 nm
- Optimal laser line
- 488
- Relative brightness
- 5 of 5
- Commercial antibodies
- 39 from 1 supplier
StarBright Blue 675 has an excitation maximum at 476 nm and an emission maximum at 675 nm, a Stokes shift of 199 nm. It is optimally excited by the 488 laser line.
Frequently asked questions
- What is StarBright Blue 675?
- StarBright Blue 675 (SBB675) Dye is a very bright, 488 nm laser excitable dye with a unique narrow excitation and emission profile and is suitable for conventional and full spectrum flow cytometry. It is brighter than other 488 nm laser excitable dyes such as PerCP-Cy5.5 (and PerCP) and BB700 and PE-Cy5 when excited by the 488 nm laser. As it is not a traditional tandem dye, SBB675 does not have issues around tandem breakdown and changes to staining over time. It exhibits reduced excitation by the 561 nm laser compared to PE tandem dyes. SBB675 provides more choice and flexibility when building multicolor panels incorporating the 488 nm laser.
- What laser excites StarBright Blue 675?
- StarBright Blue 675 is optimally excited by the 488 laser line. Its excitation maximum is 476 nm.
- How bright is StarBright Blue 675?
- StarBright Blue 675 has a relative brightness of 5 out of 5.
- How many antibodies are available conjugated to StarBright Blue 675?
- FluoroFinder tracks 39 commercially available StarBright Blue 675 conjugates, searchable by target, clone, host and application.