StarBright Blue 580
StarBright Blue 580 (Ex-Max 475 nm/Em-Max 582 nm)
StarBright Blue 580 (SBB580) Dye is a bright, 488 nm laser excitable dye with a unique narrow excitation and emission profile and is suitable for conventional and full spectrum flow cytometry. It is brighter than other 488 nm laser excitable dyes such as AF532 and is as bright as PE when excited by the 488 nm laser. It has the added benefit of having reduced excitation by the 561 nm laser compared with PE. SBB580 and PE are compatible in a panel when using both the 488 and 561 nm lasers to excite each respective dye. This provides more choice and flexibility when building multicolor panels.
Excitation & emission spectra
Key facts
- Excitation maximum
- 475 nm
- Emission maximum
- 582 nm
- Optimal laser line
- 488
- Relative brightness
- 4 of 5
- Commercial antibodies
- 40 from 1 supplier
StarBright Blue 580 has an excitation maximum at 475 nm and an emission maximum at 582 nm, a Stokes shift of 107 nm. It is optimally excited by the 488 laser line.
Frequently asked questions
- What is StarBright Blue 580?
- StarBright Blue 580 (SBB580) Dye is a bright, 488 nm laser excitable dye with a unique narrow excitation and emission profile and is suitable for conventional and full spectrum flow cytometry. It is brighter than other 488 nm laser excitable dyes such as AF532 and is as bright as PE when excited by the 488 nm laser. It has the added benefit of having reduced excitation by the 561 nm laser compared with PE. SBB580 and PE are compatible in a panel when using both the 488 and 561 nm lasers to excite each respective dye. This provides more choice and flexibility when building multicolor panels.
- What laser excites StarBright Blue 580?
- StarBright Blue 580 is optimally excited by the 488 laser line. Its excitation maximum is 475 nm.
- How bright is StarBright Blue 580?
- StarBright Blue 580 has a relative brightness of 4 out of 5.
- How many antibodies are available conjugated to StarBright Blue 580?
- FluoroFinder tracks 40 commercially available StarBright Blue 580 conjugates, searchable by target, clone, host and application.