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PhenoVue Fluo-4 AM, Calcium Indicator

PhenoVue Fluo-4 AM, Calcium Indicator (Ex-Max 495 nm/Em-Max 528 nm)

PhenoVue™ Fluo-4 AM, is permeable fluorescent Ca2+ indicator and is part of Revvity’s portfolio of cellular imaging reagents. PhenoVue Fluo-4 AM Ca2+ indicator exhibits green fluorescence and is validated for use in imaging microscopy and high-content screening applications. It exhibits a maximum excitation wavelength at 495 nm and a maximum emission wavelength of 528 nm.

Excitation & emission spectra

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Key facts

Excitation maximum
495 nm
Emission maximum
528 nm
Optimal laser line
488

PhenoVue Fluo-4 AM, Calcium Indicator has an excitation maximum at 495 nm and an emission maximum at 528 nm, a Stokes shift of 33 nm. It is optimally excited by the 488 laser line.

Frequently asked questions

What is PhenoVue Fluo-4 AM, Calcium Indicator?
PhenoVue™ Fluo-4 AM, is permeable fluorescent Ca2+ indicator and is part of Revvity’s portfolio of cellular imaging reagents. PhenoVue Fluo-4 AM Ca2+ indicator exhibits green fluorescence and is validated for use in imaging microscopy and high-content screening applications. It exhibits a maximum excitation wavelength at 495 nm and a maximum emission wavelength of 528 nm.
What laser excites PhenoVue Fluo-4 AM, Calcium Indicator?
PhenoVue Fluo-4 AM, Calcium Indicator is optimally excited by the 488 laser line. Its excitation maximum is 495 nm.