PhenoVue Cal-520 AM Bright, Calcium Indicator
PhenoVue Cal-520 AM Bright, Calcium Indicator (Ex-Max 493 nm/Em-Max 515 nm)
PhenoVue™ Cal-520 AM Bright is a green, fluorescent calcium-sensitive dye which exhibits higher brightness, shorter incubation time, improved signal-to-noise ratio, reduced leakage and higher intracellular retention compared to other existing green calcium indicators, such as PhenoVue Fura-2, Fluo-4 AM and Cal-520 AM.
Excitation & emission spectra
Key facts
- Excitation maximum
- 493 nm
- Emission maximum
- 515 nm
- Optimal laser line
- 488
PhenoVue Cal-520 AM Bright, Calcium Indicator has an excitation maximum at 493 nm and an emission maximum at 515 nm, a Stokes shift of 22 nm. It is optimally excited by the 488 laser line.
Frequently asked questions
- What is PhenoVue Cal-520 AM Bright, Calcium Indicator?
- PhenoVue™ Cal-520 AM Bright is a green, fluorescent calcium-sensitive dye which exhibits higher brightness, shorter incubation time, improved signal-to-noise ratio, reduced leakage and higher intracellular retention compared to other existing green calcium indicators, such as PhenoVue Fura-2, Fluo-4 AM and Cal-520 AM.
- What laser excites PhenoVue Cal-520 AM Bright, Calcium Indicator?
- PhenoVue Cal-520 AM Bright, Calcium Indicator is optimally excited by the 488 laser line. Its excitation maximum is 493 nm.