What laser excites Spark Red 718?

Spark Red 718 is optimally excited by the 640 laser line. Its excitation maximum is 695 nm.

What filter should I use to detect Spark Red 718?

A 695/40 filter captures the peak of the Spark Red 718 emission near 710 nm with minimal spillover.

How bright is Spark Red 718?

Spark Red 718 has a relative brightness of 3 out of 5.

How many antibodies are available conjugated to Spark Red 718?

FluoroFinder tracks 448 commercially available Spark Red 718 conjugates, searchable by target, clone, host and application.

Spark Red 718

Spark Red 718 (Ex-Max 695 nm/Em-Max 710 nm)

Spark Red™ 718 is a brighter alternative to Alexa Fluor® 700, adding to the fluorophore options for the red laser that can detect antigens with lower expression levels. It can be used on a conventional cytometer with a 633 nm red laser and 720/45 filter set (or equivalent filter used to detect Alexa Fluor® 700) spectral cytometers with appropriate instrument settings. On conventional cytometers, Spark Red™ 718 can be utilized with both APC and APC/Cyanine7. On spectral cytometers, it can be unmixed from APC, Spark NIR™ 685, Alexa Fluor® 660, APC/Cyanine7, and APC/Fire™ 810. As spectral properties of Spark Red™ 718 and Alexa Fluor® 700 are nearly identical, using them together in a multicolor panel is not recommended. With a medium brightness, Spark Red™ 718 is best suited for detecting antigens with low to moderate expression levels.

Excitation & emission spectra

Key facts

Excitation maximum: 695 nm
Emission maximum: 710 nm
Optimal laser line: 640
Optimal filter: 695/40
Relative brightness: 3 of 5
Commercial antibodies: 448 from 2 suppliers

Spark Red 718 has an excitation maximum at 695 nm and an emission maximum at 710 nm, a Stokes shift of 15 nm. It is optimally excited by the 640 laser line.

Frequently asked questions

What is Spark Red 718?: Spark Red™ 718 is a brighter alternative to Alexa Fluor® 700, adding to the fluorophore options for the red laser that can detect antigens with lower expression levels. It can be used on a conventional cytometer with a 633 nm red laser and 720/45 filter set (or equivalent filter used to detect Alexa Fluor® 700) spectral cytometers with appropriate instrument settings. On conventional cytometers, Spark Red™ 718 can be utilized with both APC and APC/Cyanine7. On spectral cytometers, it can be unmixed from APC, Spark NIR™ 685, Alexa Fluor® 660, APC/Cyanine7, and APC/Fire™ 810. As spectral properties of Spark Red™ 718 and Alexa Fluor® 700 are nearly identical, using them together in a multicolor panel is not recommended. With a medium brightness, Spark Red™ 718 is best suited for detecting antigens with low to moderate expression levels.
What laser excites Spark Red 718?: Spark Red 718 is optimally excited by the 640 laser line. Its excitation maximum is 695 nm.
What filter should I use to detect Spark Red 718?: A 695/40 filter captures the peak of the Spark Red 718 emission near 710 nm with minimal spillover.
How bright is Spark Red 718?: Spark Red 718 has a relative brightness of 3 out of 5.
How many antibodies are available conjugated to Spark Red 718?: FluoroFinder tracks 448 commercially available Spark Red 718 conjugates, searchable by target, clone, host and application.