PhenoVue DAPI Nuclear Stain
PhenoVue DAPI Nuclear Stain (Ex-Max 348 nm/Em-Max 457 nm)
PhenoVue™ DAPI nuclear stain is a cell-impermeable organic molecule that binds to DNA and is commonly used to stain cell's nucleus. Part of Revvity’s portfolio of cellular imaging reagents, the PhenoVue DAPI nuclear stain has a maximum excitation wavelength of 348nm and a maximum emission wavelength of 457nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 348 nm
- Emission maximum
- 457 nm
- Optimal laser line
- 355
- Optimal filter
- 450/50
PhenoVue DAPI Nuclear Stain has an excitation maximum at 348 nm and an emission maximum at 457 nm, a Stokes shift of 109 nm. It is optimally excited by the 355 laser line.
Frequently asked questions
- What is PhenoVue DAPI Nuclear Stain?
- PhenoVue™ DAPI nuclear stain is a cell-impermeable organic molecule that binds to DNA and is commonly used to stain cell's nucleus. Part of Revvity’s portfolio of cellular imaging reagents, the PhenoVue DAPI nuclear stain has a maximum excitation wavelength of 348nm and a maximum emission wavelength of 457nm.
- What laser excites PhenoVue DAPI Nuclear Stain?
- PhenoVue DAPI Nuclear Stain is optimally excited by the 355 laser line. Its excitation maximum is 348 nm.
- What filter should I use to detect PhenoVue DAPI Nuclear Stain?
- A 450/50 filter captures the peak of the PhenoVue DAPI Nuclear Stain emission near 457 nm with minimal spillover.