RealYellow 586
RY586 (Ex-Max 565 nm/Em-Max 586 nm)
BD Horizon RealYellow 586 (RY586) is a bright, yellow emitting dye used in flow cytometry. This fluorophore is excited optimally by the 561 laser and is similar in its emission properties to PE, but interestingly, RY586 shows minimal cross-laser excitation with the 488 laser and can be used in spectral flow cytometry panels concurrently with PE (as stated by supplier).
Excitation & emission spectra
Key facts
- Excitation maximum
- 565 nm
- Emission maximum
- 586 nm
- Optimal laser line
- 561
- Optimal filter
- 585/42
- Relative brightness
- 4 of 5
- Commercial antibodies
- 805 from 1 supplier
RealYellow 586 has an excitation maximum at 565 nm and an emission maximum at 586 nm, a Stokes shift of 21 nm. It is optimally excited by the 561 laser line.
Frequently asked questions
- What is RealYellow 586?
- BD Horizon RealYellow 586 (RY586) is a bright, yellow emitting dye used in flow cytometry. This fluorophore is excited optimally by the 561 laser and is similar in its emission properties to PE, but interestingly, RY586 shows minimal cross-laser excitation with the 488 laser and can be used in spectral flow cytometry panels concurrently with PE (as stated by supplier).
- What laser excites RealYellow 586?
- RealYellow 586 is optimally excited by the 561 laser line. Its excitation maximum is 565 nm.
- What filter should I use to detect RealYellow 586?
- A 585/42 filter captures the peak of the RealYellow 586 emission near 586 nm with minimal spillover.
- How bright is RealYellow 586?
- RealYellow 586 has a relative brightness of 4 out of 5.
- How many antibodies are available conjugated to RealYellow 586?
- FluoroFinder tracks 805 commercially available RealYellow 586 conjugates, searchable by target, clone, host and application.