PhenoVue 577 Lysosomal Stain
PhenoVue™ 577 Lysosomal Stain (Ex-Max 577 nm/Em-Max 589 nm)
PhenoVue™ 577 Lysosomal Stain from Perkin Elmer is an orange emitting dye that accumulates in acidic vesicles such as lysosomes. This dye can be used in fluorescence microscopy and high content screening applications. It has an excitation peak at 577 nm and an emission peak at 589 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 577 nm
- Emission maximum
- 589 nm
- Optimal laser line
- 561
- Optimal filter
- 585/42
PhenoVue 577 Lysosomal Stain has an excitation maximum at 577 nm and an emission maximum at 589 nm, a Stokes shift of 12 nm. It is optimally excited by the 561 laser line.
Frequently asked questions
- What is PhenoVue 577 Lysosomal Stain?
- PhenoVue™ 577 Lysosomal Stain from Perkin Elmer is an orange emitting dye that accumulates in acidic vesicles such as lysosomes. This dye can be used in fluorescence microscopy and high content screening applications. It has an excitation peak at 577 nm and an emission peak at 589 nm.
- What laser excites PhenoVue 577 Lysosomal Stain?
- PhenoVue 577 Lysosomal Stain is optimally excited by the 561 laser line. Its excitation maximum is 577 nm.
- What filter should I use to detect PhenoVue 577 Lysosomal Stain?
- A 585/42 filter captures the peak of the PhenoVue 577 Lysosomal Stain emission near 589 nm with minimal spillover.