Spark Violet 423
Spark Violet 423 (Ex-Max 399 nm/Em-Max 414 nm)
Spark Violet 423 from Biolegend is a fluorophore designed for spectral flow cytometry with an excitation peak at 400 nm and an emission peak at 423 nm. This fluorophore is an alternative to BV421 and can be excited by the 405 nm laser.
Excitation & emission spectra
Key facts
- Excitation maximum
- 399 nm
- Emission maximum
- 414 nm
- Optimal laser line
- 405
- Optimal filter
- 410/20
- Relative brightness
- 2 of 5
- Commercial antibodies
- 178 from 2 suppliers
Spark Violet 423 has an excitation maximum at 399 nm and an emission maximum at 414 nm, a Stokes shift of 15 nm. It is optimally excited by the 405 laser line.
Frequently asked questions
- What is Spark Violet 423?
- Spark Violet 423 from Biolegend is a fluorophore designed for spectral flow cytometry with an excitation peak at 400 nm and an emission peak at 423 nm. This fluorophore is an alternative to BV421 and can be excited by the 405 nm laser.
- What laser excites Spark Violet 423?
- Spark Violet 423 is optimally excited by the 405 laser line. Its excitation maximum is 399 nm.
- What filter should I use to detect Spark Violet 423?
- A 410/20 filter captures the peak of the Spark Violet 423 emission near 414 nm with minimal spillover.
- How bright is Spark Violet 423?
- Spark Violet 423 has a relative brightness of 2 out of 5.
- How many antibodies are available conjugated to Spark Violet 423?
- FluoroFinder tracks 178 commercially available Spark Violet 423 conjugates, searchable by target, clone, host and application.