NucSpot 680
NucSpot 680 (Ex-Max 685 nm/Em-Max 708 nm)
NucSpot 680/700 is a cell-impermeant, fluorogenic nucleic acid stain used live/dead discrimination or counterstaining fixed-cells. It has an excitation peak at 685 nm and an emission peak at 708 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 685 nm
- Emission maximum
- 708 nm
- Optimal laser line
- 628-640
- Optimal filter
- 695/40
NucSpot 680 has an excitation maximum at 685 nm and an emission maximum at 708 nm, a Stokes shift of 23 nm. It is optimally excited by the 628-640 laser line.
Frequently asked questions
- What is NucSpot 680?
- NucSpot 680/700 is a cell-impermeant, fluorogenic nucleic acid stain used live/dead discrimination or counterstaining fixed-cells. It has an excitation peak at 685 nm and an emission peak at 708 nm.
- What laser excites NucSpot 680?
- NucSpot 680 is optimally excited by the 628-640 laser line. Its excitation maximum is 685 nm.
- What filter should I use to detect NucSpot 680?
- A 695/40 filter captures the peak of the NucSpot 680 emission near 708 nm with minimal spillover.