Cascade Yellow
Cascade Yellow (Ex-Max 402 nm/Em-Max 545 nm)
Cascade Yellow has an excitation peak at 402 nm and an emission peak at 545 nm. It is spectrally similar to Krome Orange and Pacific Orange. A superior alternative to Cascade Yellow in flow cytometry and fluorescence microscopy is Brilliant™ Violet 510, StarBright Violet 515 and Brilliant™ Violet 480.
Excitation & emission spectra
Key facts
- Excitation maximum
- 402 nm
- Emission maximum
- 545 nm
- Optimal laser line
- 405
- Optimal filter
- 550/40
- Relative brightness
- 1 of 5
- Extinction coefficient
- 25000
- Quantum yield
- 0.56
Cascade Yellow has an excitation maximum at 402 nm and an emission maximum at 545 nm, a Stokes shift of 143 nm. Its extinction coefficient is 25000 M⁻¹cm⁻¹. It is optimally excited by the 405 laser line.
Frequently asked questions
- What is Cascade Yellow?
- Cascade Yellow has an excitation peak at 402 nm and an emission peak at 545 nm. It is spectrally similar to Krome Orange and Pacific Orange. A superior alternative to Cascade Yellow in flow cytometry and fluorescence microscopy is Brilliant™ Violet 510, StarBright Violet 515 and Brilliant™ Violet 480.
- What laser excites Cascade Yellow?
- Cascade Yellow is optimally excited by the 405 laser line. Its excitation maximum is 402 nm.
- What filter should I use to detect Cascade Yellow?
- A 550/40 filter captures the peak of the Cascade Yellow emission near 545 nm with minimal spillover.
- How bright is Cascade Yellow?
- Cascade Yellow has a relative brightness of 1 out of 5 (quantum yield 0.56).