LysoView 594
LysoView™ 594 (Ex-Max 585 nm/Em-Max 634 nm)
Biotium's LysoView™ dyes are cell-permeant alkaline probes that localize to acidic compartments inside the cell, such as endosomes and lysosomes. These types of probes are designed for live-cell imaging and do not typically survive fixation and permeabilization. LysoView™ 594 has an excitation peak at 585 nm and an emission peak at 634 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 585 nm
- Emission maximum
- 634 nm
- Optimal laser line
- 561
- Optimal filter
- 630/69
- Commercial antibodies
- 2 from 1 supplier
LysoView 594 has an excitation maximum at 585 nm and an emission maximum at 634 nm, a Stokes shift of 49 nm. It is optimally excited by the 561 laser line.
Frequently asked questions
- What is LysoView 594?
- Biotium's LysoView™ dyes are cell-permeant alkaline probes that localize to acidic compartments inside the cell, such as endosomes and lysosomes. These types of probes are designed for live-cell imaging and do not typically survive fixation and permeabilization. LysoView™ 594 has an excitation peak at 585 nm and an emission peak at 634 nm.
- What laser excites LysoView 594?
- LysoView 594 is optimally excited by the 561 laser line. Its excitation maximum is 585 nm.
- What filter should I use to detect LysoView 594?
- A 630/69 filter captures the peak of the LysoView 594 emission near 634 nm with minimal spillover.
- How many antibodies are available conjugated to LysoView 594?
- FluoroFinder tracks 2 commercially available LysoView 594 conjugates, searchable by target, clone, host and application.