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Oxazole Red

Oxazole Red (Ex-Max 613 nm/Em-Max 629 nm)

Oxazole Red (YO-PRO-3) is a cell-impermeant, fluorogenic intercalating nucleic acid stain. Oxazole Red has an excitation peak at 613 nm and an emission peak at 629 nm. It is commonly used as a dead and apoptotic cell stain in the following applications: Fluorescence Microscopy and Flow Cytometry.

Excitation & emission spectra

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Key facts

Excitation maximum
613 nm
Emission maximum
629 nm
Optimal laser line
594
Optimal filter
630/69

Oxazole Red has an excitation maximum at 613 nm and an emission maximum at 629 nm, a Stokes shift of 16 nm. It is optimally excited by the 594 laser line.

Frequently asked questions

What is Oxazole Red?
Oxazole Red (YO-PRO-3) is a cell-impermeant, fluorogenic intercalating nucleic acid stain. Oxazole Red has an excitation peak at 613 nm and an emission peak at 629 nm. It is commonly used as a dead and apoptotic cell stain in the following applications: Fluorescence Microscopy and Flow Cytometry.
What laser excites Oxazole Red?
Oxazole Red is optimally excited by the 594 laser line. Its excitation maximum is 613 nm.
What filter should I use to detect Oxazole Red?
A 630/69 filter captures the peak of the Oxazole Red emission near 629 nm with minimal spillover.