What laser excites PE-Fire 810?

PE-Fire 810 is optimally excited by the 561 laser line. Its excitation maximum is 565 nm.

What filter should I use to detect PE-Fire 810?

A 780/60 filter captures the peak of the PE-Fire 810 emission near 810 nm with minimal spillover.

How bright is PE-Fire 810?

PE-Fire 810 has a relative brightness of 4 out of 5.

How many antibodies are available conjugated to PE-Fire 810?

FluoroFinder tracks 187 commercially available PE-Fire 810 conjugates, searchable by target, clone, host and application.

PE-Fire 810

PE-Fire 810 (Ex-Max 565 nm/Em-Max 810 nm)

Along with APC/Fire 810™, PE/Fire™ 810 expands the range of spectral detection further than any of our previous fluorophores, emitting even beyond the range of PE/Cyanine7. Optimized for spectral unmixing cytometers, PE/Fire™ 810 is excited by the blue and yellow/green lasers and can utilized for nearly any antigen since it has little spectral overlap with other fluorophores. While we have released several PE tandem options, our data below shows PE/Fire™ 810 can be unmixed from them in a multicolor panel.

Excitation & emission spectra

Key facts

Excitation maximum: 565 nm
Emission maximum: 810 nm
Optimal laser line: 561
Optimal filter: 780/60
Relative brightness: 4 of 5
Commercial antibodies: 187 from 2 suppliers

PE-Fire 810 has an excitation maximum at 565 nm and an emission maximum at 810 nm, a Stokes shift of 245 nm. It is optimally excited by the 561 laser line.

Frequently asked questions

What is PE-Fire 810?: Along with APC/Fire 810™, PE/Fire™ 810 expands the range of spectral detection further than any of our previous fluorophores, emitting even beyond the range of PE/Cyanine7. Optimized for spectral unmixing cytometers, PE/Fire™ 810 is excited by the blue and yellow/green lasers and can utilized for nearly any antigen since it has little spectral overlap with other fluorophores. While we have released several PE tandem options, our data below shows PE/Fire™ 810 can be unmixed from them in a multicolor panel.
What laser excites PE-Fire 810?: PE-Fire 810 is optimally excited by the 561 laser line. Its excitation maximum is 565 nm.
What filter should I use to detect PE-Fire 810?: A 780/60 filter captures the peak of the PE-Fire 810 emission near 810 nm with minimal spillover.
How bright is PE-Fire 810?: PE-Fire 810 has a relative brightness of 4 out of 5.
How many antibodies are available conjugated to PE-Fire 810?: FluoroFinder tracks 187 commercially available PE-Fire 810 conjugates, searchable by target, clone, host and application.