Live/Dead Fix Near IR (876)
Live/Dead Fix Near IR (876) (Ex-Max 834 nm/Em-Max 870 nm)
The Live/Dead Fixable Viability probes from Thermo Fisher are amine-reactive and used for live/dead cell discrimination in flow cytometry. These probes pass through the permeable cell membrane of dead cells to stain intracellular amine groups. However, the probe cannot pass through the intact membranes of live cells. Additionally, these dyes are very stable and can withstand fixation, permeabilization, and will not transfer between cells. Live/Dead Fixable Near IR (876) has an excitation peak at 834 nm and an emission peak at 870 nm. This viability dye is designed for flow cytometers with a 785 or 808 nm excitation laser.
Excitation & emission spectra
Key facts
- Excitation maximum
- 834 nm
- Emission maximum
- 870 nm
- Optimal laser line
- 785
- Optimal filter
- 885/40
- Commercial antibodies
- 3 from 1 supplier
Live/Dead Fix Near IR (876) has an excitation maximum at 834 nm and an emission maximum at 870 nm, a Stokes shift of 36 nm. It is optimally excited by the 785 laser line.
Frequently asked questions
- What is Live/Dead Fix Near IR (876)?
- The Live/Dead Fixable Viability probes from Thermo Fisher are amine-reactive and used for live/dead cell discrimination in flow cytometry. These probes pass through the permeable cell membrane of dead cells to stain intracellular amine groups. However, the probe cannot pass through the intact membranes of live cells. Additionally, these dyes are very stable and can withstand fixation, permeabilization, and will not transfer between cells. Live/Dead Fixable Near IR (876) has an excitation peak at 834 nm and an emission peak at 870 nm. This viability dye is designed for flow cytometers with a 785 or 808 nm excitation laser.
- What laser excites Live/Dead Fix Near IR (876)?
- Live/Dead Fix Near IR (876) is optimally excited by the 785 laser line. Its excitation maximum is 834 nm.
- What filter should I use to detect Live/Dead Fix Near IR (876)?
- A 885/40 filter captures the peak of the Live/Dead Fix Near IR (876) emission near 870 nm with minimal spillover.
- How many antibodies are available conjugated to Live/Dead Fix Near IR (876)?
- FluoroFinder tracks 3 commercially available Live/Dead Fix Near IR (876) conjugates, searchable by target, clone, host and application.