Live/Dead Fix Orange
Live/Dead Fix Orange (Ex-Max 575 nm/Em-Max 601 nm)
The Live/Dead Fixable Viability probes from Thermo Fisher are amine-reactive and used for live/dead cell discrimination in flow cytometry. These probes pass through the permeable cell membrane of dead cells to stain intracellular amine groups. However, the probe cannot pass through the intact membranes of live cells. Additionally, these dyes are very stable and can withstand fixation, permeabilization, and will not transfer between cells. Live/Dead Fixable Orange has an excitation peak at 575 nm and an emission peak at 601 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 575 nm
- Emission maximum
- 601 nm
- Optimal laser line
- 561
- Optimal filter
- 610/20
- Commercial antibodies
- 3 from 1 supplier
Live/Dead Fix Orange has an excitation maximum at 575 nm and an emission maximum at 601 nm, a Stokes shift of 26 nm. It is optimally excited by the 561 laser line.
Frequently asked questions
- What is Live/Dead Fix Orange?
- The Live/Dead Fixable Viability probes from Thermo Fisher are amine-reactive and used for live/dead cell discrimination in flow cytometry. These probes pass through the permeable cell membrane of dead cells to stain intracellular amine groups. However, the probe cannot pass through the intact membranes of live cells. Additionally, these dyes are very stable and can withstand fixation, permeabilization, and will not transfer between cells. Live/Dead Fixable Orange has an excitation peak at 575 nm and an emission peak at 601 nm.
- What laser excites Live/Dead Fix Orange?
- Live/Dead Fix Orange is optimally excited by the 561 laser line. Its excitation maximum is 575 nm.
- What filter should I use to detect Live/Dead Fix Orange?
- A 610/20 filter captures the peak of the Live/Dead Fix Orange emission near 601 nm with minimal spillover.
- How many antibodies are available conjugated to Live/Dead Fix Orange?
- FluoroFinder tracks 3 commercially available Live/Dead Fix Orange conjugates, searchable by target, clone, host and application.