Live/Dead Fix Olive
Live/Dead Fix Olive (Ex-Max 475 nm/Em-Max 560 nm)
The Live/Dead Fixable Viability probes from Thermo Fisher are amine-reactive and used for live/dead cell discrimination in flow cytometry. These probes pass through the permeable cell membrane of dead cells to stain intracellular amine groups. However, the probe cannot pass through the intact membranes of live cells. Additionally, these dyes are very stable and can withstand fixation, permeabilization, and will not transfer between cells. Live/Dead Fixable Olive has an excitation peak at 475 nm and an emission peak at 560 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 475 nm
- Emission maximum
- 560 nm
- Optimal laser line
- 488
- Optimal filter
- 550/40
- Commercial antibodies
- 3 from 1 supplier
Live/Dead Fix Olive has an excitation maximum at 475 nm and an emission maximum at 560 nm, a Stokes shift of 85 nm. It is optimally excited by the 488 laser line.
Frequently asked questions
- What is Live/Dead Fix Olive?
- The Live/Dead Fixable Viability probes from Thermo Fisher are amine-reactive and used for live/dead cell discrimination in flow cytometry. These probes pass through the permeable cell membrane of dead cells to stain intracellular amine groups. However, the probe cannot pass through the intact membranes of live cells. Additionally, these dyes are very stable and can withstand fixation, permeabilization, and will not transfer between cells. Live/Dead Fixable Olive has an excitation peak at 475 nm and an emission peak at 560 nm.
- What laser excites Live/Dead Fix Olive?
- Live/Dead Fix Olive is optimally excited by the 488 laser line. Its excitation maximum is 475 nm.
- What filter should I use to detect Live/Dead Fix Olive?
- A 550/40 filter captures the peak of the Live/Dead Fix Olive emission near 560 nm with minimal spillover.
- How many antibodies are available conjugated to Live/Dead Fix Olive?
- FluoroFinder tracks 3 commercially available Live/Dead Fix Olive conjugates, searchable by target, clone, host and application.