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iFluor 820

iFluor® 820 (Ex-Max 820 nm/Em-Max 850 nm)

iFluor® 820 from AAT Bioquest is a fluorophore with an excitation peak at 820 nm and an emission peak at 850 nm. It can be useful in fluorescence and in vivo fluorescence microscopy. It may also be useful in flow cytometry when excited by an 808 nm laser.

Excitation & emission spectra

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Key facts

Excitation maximum
820 nm
Emission maximum
850 nm
Optimal laser line
808
Optimal filter
810LP

iFluor 820 has an excitation maximum at 820 nm and an emission maximum at 850 nm, a Stokes shift of 30 nm. It is optimally excited by the 808 laser line.

Frequently asked questions

What is iFluor 820?
iFluor® 820 from AAT Bioquest is a fluorophore with an excitation peak at 820 nm and an emission peak at 850 nm. It can be useful in fluorescence and in vivo fluorescence microscopy. It may also be useful in flow cytometry when excited by an 808 nm laser.
What laser excites iFluor 820?
iFluor 820 is optimally excited by the 808 laser line. Its excitation maximum is 820 nm.
What filter should I use to detect iFluor 820?
A 810LP filter captures the peak of the iFluor 820 emission near 850 nm with minimal spillover.