PhenoVue 512 Nucleic Acid Stain – complexed with nucleic acids
PhenoVue 512 Nucleic Acid Stain – complexed with nucleic acids (Ex-Max 516 nm/Em-Max 556 nm)
PhenoVue™ 512 nucleic acid stain is a cell permeable organic molecule which binds nucleic acids. It displays a higher fluorescence intensity when complexed with RNA compared to DNA. PhenoVue 512 nucleic acid stain concentrates into RNA enriched organelles, such as the membraneless organelles (MLOs), nucleoli, found in the nuclei of mammalian cells. Therefore, PhenoVue 512 nucleic acid stain is the dye of choice for nucleoli staining.
Excitation & emission spectra
Key facts
- Excitation maximum
- 516 nm
- Emission maximum
- 556 nm
- Optimal laser line
- 488
- Optimal filter
- 515/30
PhenoVue 512 Nucleic Acid Stain – complexed with nucleic acids has an excitation maximum at 516 nm and an emission maximum at 556 nm, a Stokes shift of 40 nm. It is optimally excited by the 488 laser line.
Frequently asked questions
- What is PhenoVue 512 Nucleic Acid Stain – complexed with nucleic acids?
- PhenoVue™ 512 nucleic acid stain is a cell permeable organic molecule which binds nucleic acids. It displays a higher fluorescence intensity when complexed with RNA compared to DNA. PhenoVue 512 nucleic acid stain concentrates into RNA enriched organelles, such as the membraneless organelles (MLOs), nucleoli, found in the nuclei of mammalian cells. Therefore, PhenoVue 512 nucleic acid stain is the dye of choice for nucleoli staining.
- What laser excites PhenoVue 512 Nucleic Acid Stain – complexed with nucleic acids?
- PhenoVue 512 Nucleic Acid Stain – complexed with nucleic acids is optimally excited by the 488 laser line. Its excitation maximum is 516 nm.
- What filter should I use to detect PhenoVue 512 Nucleic Acid Stain – complexed with nucleic acids?
- A 515/30 filter captures the peak of the PhenoVue 512 Nucleic Acid Stain – complexed with nucleic acids emission near 556 nm with minimal spillover.