mCerulean3
mCerulean3 (Ex-Max 430 nm/Em-Max 470 nm)
<html>mCerulean3 is a monomeric fluorescent protein derived from the jellyfish, <i>Aequorea victoria.</i> It has an excitation peak at 433 nm and an emission peak at 475 nm.</html>
Excitation & emission spectra
Key facts
- Excitation maximum
- 433 nm
- Emission maximum
- 475 nm
- Optimal laser line
- 405
- Optimal filter
- 450/50
- Extinction coefficient
- 40000
- Quantum yield
- 0.87
mCerulean3 has an excitation maximum at 433 nm and an emission maximum at 475 nm, a Stokes shift of 42 nm. Its extinction coefficient is 40000 M⁻¹cm⁻¹. It is optimally excited by the 405 laser line.
Frequently asked questions
- What is mCerulean3?
- <html>mCerulean3 is a monomeric fluorescent protein derived from the jellyfish, <i>Aequorea victoria.</i> It has an excitation peak at 433 nm and an emission peak at 475 nm.</html>
- What laser excites mCerulean3?
- mCerulean3 is optimally excited by the 405 laser line. Its excitation maximum is 433 nm.
- What filter should I use to detect mCerulean3?
- A 450/50 filter captures the peak of the mCerulean3 emission near 475 nm with minimal spillover.