Spark Violet 538
Spark Violet 538 (Ex-Max 397 nm/Em-Max 538 nm)
Spark Violet 538 from Biolegend is a fluorophore designed for spectral flow cytometry with an excitation peak at 399 nm and an emission peak at 538 nm. This fluorophore is an alternative to Pacific Orange and can be efficiently excited by the 405 nm laser.
Excitation & emission spectra
Key facts
- Excitation maximum
- 397 nm
- Emission maximum
- 538 nm
- Optimal laser line
- 405
- Optimal filter
- 525/50
- Relative brightness
- 1 of 5
- Commercial antibodies
- 118 from 2 suppliers
Spark Violet 538 has an excitation maximum at 397 nm and an emission maximum at 538 nm, a Stokes shift of 141 nm. It is optimally excited by the 405 laser line.
Frequently asked questions
- What is Spark Violet 538?
- Spark Violet 538 from Biolegend is a fluorophore designed for spectral flow cytometry with an excitation peak at 399 nm and an emission peak at 538 nm. This fluorophore is an alternative to Pacific Orange and can be efficiently excited by the 405 nm laser.
- What laser excites Spark Violet 538?
- Spark Violet 538 is optimally excited by the 405 laser line. Its excitation maximum is 397 nm.
- What filter should I use to detect Spark Violet 538?
- A 525/50 filter captures the peak of the Spark Violet 538 emission near 538 nm with minimal spillover.
- How bright is Spark Violet 538?
- Spark Violet 538 has a relative brightness of 1 out of 5.
- How many antibodies are available conjugated to Spark Violet 538?
- FluoroFinder tracks 118 commercially available Spark Violet 538 conjugates, searchable by target, clone, host and application.