What laser excites Brilliant Ultraviolet 615?

Brilliant Ultraviolet 615 is optimally excited by the 355 laser line. Its excitation maximum is 348 nm.

What filter should I use to detect Brilliant Ultraviolet 615?

A 610/20 filter captures the peak of the Brilliant Ultraviolet 615 emission near 616 nm with minimal spillover.

How bright is Brilliant Ultraviolet 615?

Brilliant Ultraviolet 615 has a relative brightness of 3 out of 5.

How many antibodies are available conjugated to Brilliant Ultraviolet 615?

FluoroFinder tracks 963 commercially available Brilliant Ultraviolet 615 conjugates, searchable by target, clone, host and application.

Brilliant Ultraviolet 615

BUV615 (Ex-Max 350 nm/Em-Max 616 nm)

BD Horizon Brilliant™ UltraViolet 615 (BUV615) is a tandem dye has an excitation peak at 348 nm and has an emission maximum of 616 nm. This dye is part of the Brilliant™ UltraViolet dye family, developed and sold by BD Biosciences. This family of dyes uses BUV395 as the donor so that all dyes in the family can be excited by the 355 nm UV laser. Depending on the acceptor, the dyes in this family have different relative brightness ratings.

Excitation & emission spectra

Key facts

Excitation maximum: 348 nm
Emission maximum: 616 nm
Optimal laser line: 355
Optimal filter: 610/20
Relative brightness: 3 of 5
Commercial antibodies: 963 from 2 suppliers

Brilliant Ultraviolet 615 has an excitation maximum at 348 nm and an emission maximum at 616 nm, a Stokes shift of 268 nm. It is optimally excited by the 355 laser line.

Frequently asked questions

What is Brilliant Ultraviolet 615?: BD Horizon Brilliant™ UltraViolet 615 (BUV615) is a tandem dye has an excitation peak at 348 nm and has an emission maximum of 616 nm. This dye is part of the Brilliant™ UltraViolet dye family, developed and sold by BD Biosciences. This family of dyes uses BUV395 as the donor so that all dyes in the family can be excited by the 355 nm UV laser. Depending on the acceptor, the dyes in this family have different relative brightness ratings.
What laser excites Brilliant Ultraviolet 615?: Brilliant Ultraviolet 615 is optimally excited by the 355 laser line. Its excitation maximum is 348 nm.
What filter should I use to detect Brilliant Ultraviolet 615?: A 610/20 filter captures the peak of the Brilliant Ultraviolet 615 emission near 616 nm with minimal spillover.
How bright is Brilliant Ultraviolet 615?: Brilliant Ultraviolet 615 has a relative brightness of 3 out of 5.
How many antibodies are available conjugated to Brilliant Ultraviolet 615?: FluoroFinder tracks 963 commercially available Brilliant Ultraviolet 615 conjugates, searchable by target, clone, host and application.