PerCP-eFluor 710
PerCP-eFluor™ 710 (Ex-Max 482 nm/Em-Max 710 nm)
PerCP-eFluor™ 710 (PerCP-eF710) is a red-emitting tandem fluorophore that combines PerCP and eF710. The donor molecule, PerCP can be excited by the 488-nm blue laser and and transfers energy to the acceptor molecule, eF710, which emitts light that can be captured with a 695/40 nm bandpass filter. PerCP-eF710 has an excitation peak at 482 nm and an emission peak at 710 nm, and is considered a slightly-red shifted but brighter alternative to PerCP-Cy5.5. This dye and associated products are mostly used for flow cytometry. This dye is part of the larger eFluor™ dye family, developed by eBioscience which is now part of ThermoFisher Scientific.
Excitation & emission spectra
Key facts
- Excitation maximum
- 482 nm
- Emission maximum
- 710 nm
- Optimal laser line
- 488
- Optimal filter
- 695/40
- Relative brightness
- 3 of 5
- Commercial antibodies
- 700 from 3 suppliers
PerCP-eFluor 710 has an excitation maximum at 482 nm and an emission maximum at 710 nm, a Stokes shift of 228 nm. It is optimally excited by the 488 laser line.
Frequently asked questions
- What is PerCP-eFluor 710?
- PerCP-eFluor™ 710 (PerCP-eF710) is a red-emitting tandem fluorophore that combines PerCP and eF710. The donor molecule, PerCP can be excited by the 488-nm blue laser and and transfers energy to the acceptor molecule, eF710, which emitts light that can be captured with a 695/40 nm bandpass filter. PerCP-eF710 has an excitation peak at 482 nm and an emission peak at 710 nm, and is considered a slightly-red shifted but brighter alternative to PerCP-Cy5.5. This dye and associated products are mostly used for flow cytometry. This dye is part of the larger eFluor™ dye family, developed by eBioscience which is now part of ThermoFisher Scientific.
- What laser excites PerCP-eFluor 710?
- PerCP-eFluor 710 is optimally excited by the 488 laser line. Its excitation maximum is 482 nm.
- What filter should I use to detect PerCP-eFluor 710?
- A 695/40 filter captures the peak of the PerCP-eFluor 710 emission near 710 nm with minimal spillover.
- How bright is PerCP-eFluor 710?
- PerCP-eFluor 710 has a relative brightness of 3 out of 5.
- How many antibodies are available conjugated to PerCP-eFluor 710?
- FluoroFinder tracks 700 commercially available PerCP-eFluor 710 conjugates, searchable by target, clone, host and application.