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PhenoVue Hoechst 33342 Nuclear Stain

PhenoVue™ Hoechst 33342 Nuclear Stain (Ex-Max 352 nm/Em-Max 453 nm)

PhenoVue™ Hoechst 33342 Nuclear Stain from Perkin Elmer is a cell-permeable dye that binds to A-T regions in DNA. It has an excitation peak at 352 nm and an emission peak at 453 nm.

Excitation & emission spectra

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Key facts

Excitation maximum
352 nm
Emission maximum
453 nm
Optimal laser line
355
Optimal filter
450/50
Commercial antibodies
2 from 1 supplier

PhenoVue Hoechst 33342 Nuclear Stain has an excitation maximum at 352 nm and an emission maximum at 453 nm, a Stokes shift of 101 nm. It is optimally excited by the 355 laser line.

Frequently asked questions

What is PhenoVue Hoechst 33342 Nuclear Stain?
PhenoVue™ Hoechst 33342 Nuclear Stain from Perkin Elmer is a cell-permeable dye that binds to A-T regions in DNA. It has an excitation peak at 352 nm and an emission peak at 453 nm.
What laser excites PhenoVue Hoechst 33342 Nuclear Stain?
PhenoVue Hoechst 33342 Nuclear Stain is optimally excited by the 355 laser line. Its excitation maximum is 352 nm.
What filter should I use to detect PhenoVue Hoechst 33342 Nuclear Stain?
A 450/50 filter captures the peak of the PhenoVue Hoechst 33342 Nuclear Stain emission near 453 nm with minimal spillover.
How many antibodies are available conjugated to PhenoVue Hoechst 33342 Nuclear Stain?
FluoroFinder tracks 2 commercially available PhenoVue Hoechst 33342 Nuclear Stain conjugates, searchable by target, clone, host and application.