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iFluor 750

iFluor® 750 (Ex-Max 755 nm/Em-Max 777 nm)

iFluor® 750 from AAT Bioquest is a fluorophore with an excitation peak at 755 nm and an emission peak at 777 nm. It can be useful in fluorescence microscopy. Spectrally, it is similar to Alexa Fluor™ 750.

Excitation & emission spectra

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Key facts

Excitation maximum
755 nm
Emission maximum
777 nm
Optimal laser line
730-750
Optimal filter
780/60
Relative brightness
1 of 5

iFluor 750 has an excitation maximum at 755 nm and an emission maximum at 777 nm, a Stokes shift of 22 nm. It is optimally excited by the 730-750 laser line.

Frequently asked questions

What is iFluor 750?
iFluor® 750 from AAT Bioquest is a fluorophore with an excitation peak at 755 nm and an emission peak at 777 nm. It can be useful in fluorescence microscopy. Spectrally, it is similar to Alexa Fluor™ 750.
What laser excites iFluor 750?
iFluor 750 is optimally excited by the 730-750 laser line. Its excitation maximum is 755 nm.
What filter should I use to detect iFluor 750?
A 780/60 filter captures the peak of the iFluor 750 emission near 777 nm with minimal spillover.
How bright is iFluor 750?
iFluor 750 has a relative brightness of 1 out of 5.