CytoTrace Red
CytoTrace Red (Ex-Max 560 nm/Em-Max 575 nm)
CytoTrace Red CFDA from AAT Bioquest loads passively into live cells where its retained by the action of intracellular esterases.
Excitation & emission spectra
Key facts
- Excitation maximum
- 560 nm
- Emission maximum
- 575 nm
- Optimal laser line
- 561
- Optimal filter
- 585/42
CytoTrace Red has an excitation maximum at 560 nm and an emission maximum at 575 nm, a Stokes shift of 15 nm. It is optimally excited by the 561 laser line.
Frequently asked questions
- What is CytoTrace Red?
- CytoTrace Red CFDA from AAT Bioquest loads passively into live cells where its retained by the action of intracellular esterases.
- What laser excites CytoTrace Red?
- CytoTrace Red is optimally excited by the 561 laser line. Its excitation maximum is 560 nm.
- What filter should I use to detect CytoTrace Red?
- A 585/42 filter captures the peak of the CytoTrace Red emission near 575 nm with minimal spillover.