iFluor 633
iFluor® 633 (Ex-Max 640 nm/Em-Max 655 nm)
iFluor® 633 is from AAT Bioquest is a fluorophore with an excitation peak at 640 nm and an emission peak at 655 nm. It can be useful in both microscopy and flow cytometry. Spectrally, it is similar to Alexa Fluor™ 633 and Cy5.
Excitation & emission spectra
Key facts
- Excitation maximum
- 640 nm
- Emission maximum
- 655 nm
- Optimal laser line
- 628-640
- Optimal filter
- 660/20
- Relative brightness
- 3 of 5
- Commercial antibodies
- 40 from 1 supplier
iFluor 633 has an excitation maximum at 640 nm and an emission maximum at 655 nm, a Stokes shift of 15 nm. It is optimally excited by the 628-640 laser line.
Frequently asked questions
- What is iFluor 633?
- iFluor® 633 is from AAT Bioquest is a fluorophore with an excitation peak at 640 nm and an emission peak at 655 nm. It can be useful in both microscopy and flow cytometry. Spectrally, it is similar to Alexa Fluor™ 633 and Cy5.
- What laser excites iFluor 633?
- iFluor 633 is optimally excited by the 628-640 laser line. Its excitation maximum is 640 nm.
- What filter should I use to detect iFluor 633?
- A 660/20 filter captures the peak of the iFluor 633 emission near 655 nm with minimal spillover.
- How bright is iFluor 633?
- iFluor 633 has a relative brightness of 3 out of 5.
- How many antibodies are available conjugated to iFluor 633?
- FluoroFinder tracks 40 commercially available iFluor 633 conjugates, searchable by target, clone, host and application.