iFluor 532
iFluor® 532 (Ex-Max 534 nm/Em-Max 558 nm)
iFluor® 532 is from AAT Bioquest is a fluorophore with an excitation peak at 534 nm and an emission peak at 558 nm. It can be useful in both microscopy and flow cytometry. Spectrally, it is similar to Alexa Fluor™ 532 .
Excitation & emission spectra
Key facts
- Excitation maximum
- 534 nm
- Emission maximum
- 558 nm
- Optimal laser line
- 532
- Optimal filter
- 550/40
- Relative brightness
- 1 of 5
iFluor 532 has an excitation maximum at 534 nm and an emission maximum at 558 nm, a Stokes shift of 24 nm. It is optimally excited by the 532 laser line.
Frequently asked questions
- What is iFluor 532?
- iFluor® 532 is from AAT Bioquest is a fluorophore with an excitation peak at 534 nm and an emission peak at 558 nm. It can be useful in both microscopy and flow cytometry. Spectrally, it is similar to Alexa Fluor™ 532 .
- What laser excites iFluor 532?
- iFluor 532 is optimally excited by the 532 laser line. Its excitation maximum is 534 nm.
- What filter should I use to detect iFluor 532?
- A 550/40 filter captures the peak of the iFluor 532 emission near 558 nm with minimal spillover.
- How bright is iFluor 532?
- iFluor 532 has a relative brightness of 1 out of 5.