iFluor 405
iFluor® 405 (Ex-Max 400 nm/Em-Max 426 nm)
iFluor® 405 from AAT Bioquest is a fluorophore with an excitation peak at 400 nm and an emission peak at 426 nm. It can be useful in both microscopy and flow cytometry. Spectrally, it is similar to Alexa Fluor™ 405 and Pacific Blue.
Excitation & emission spectra
Key facts
- Excitation maximum
- 400 nm
- Emission maximum
- 426 nm
- Optimal laser line
- 405
- Optimal filter
- 450/50
- Relative brightness
- 2 of 5
- Commercial antibodies
- 4 from 2 suppliers
iFluor 405 has an excitation maximum at 400 nm and an emission maximum at 426 nm, a Stokes shift of 26 nm. It is optimally excited by the 405 laser line.
Frequently asked questions
- What is iFluor 405?
- iFluor® 405 from AAT Bioquest is a fluorophore with an excitation peak at 400 nm and an emission peak at 426 nm. It can be useful in both microscopy and flow cytometry. Spectrally, it is similar to Alexa Fluor™ 405 and Pacific Blue.
- What laser excites iFluor 405?
- iFluor 405 is optimally excited by the 405 laser line. Its excitation maximum is 400 nm.
- What filter should I use to detect iFluor 405?
- A 450/50 filter captures the peak of the iFluor 405 emission near 426 nm with minimal spillover.
- How bright is iFluor 405?
- iFluor 405 has a relative brightness of 2 out of 5.
- How many antibodies are available conjugated to iFluor 405?
- FluoroFinder tracks 4 commercially available iFluor 405 conjugates, searchable by target, clone, host and application.