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Nuclear Yellow

Nuclear Yellow (Ex-Max 370 nm/Em-Max 504 nm)

Nuclear Yellow (Hoechst S769121) is a cell-permeant, fluorogenic DNA binding dye with an excitation peak at 372 nm and an emission peak at 504 nm. Nuclear Yellow can be used to convert DAB to an electron dense reaction product for electron microscopy. It is used in the following applications: Flow Cytometry, Fluorescence Microscopy.

Excitation & emission spectra

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Key facts

Excitation maximum
370 nm
Emission maximum
504 nm
Optimal laser line
355
Optimal filter
515/30
Molecular weight
596.96
Commercial antibodies
1 from 1 supplier

Nuclear Yellow has an excitation maximum at 370 nm and an emission maximum at 504 nm, a Stokes shift of 134 nm. It is optimally excited by the 355 laser line.

Frequently asked questions

What is Nuclear Yellow?
Nuclear Yellow (Hoechst S769121) is a cell-permeant, fluorogenic DNA binding dye with an excitation peak at 372 nm and an emission peak at 504 nm. Nuclear Yellow can be used to convert DAB to an electron dense reaction product for electron microscopy. It is used in the following applications: Flow Cytometry, Fluorescence Microscopy.
What laser excites Nuclear Yellow?
Nuclear Yellow is optimally excited by the 355 laser line. Its excitation maximum is 370 nm.
What filter should I use to detect Nuclear Yellow?
A 515/30 filter captures the peak of the Nuclear Yellow emission near 504 nm with minimal spillover.
How many antibodies are available conjugated to Nuclear Yellow?
FluoroFinder tracks 1 commercially available Nuclear Yellow conjugates, searchable by target, clone, host and application.