Nuclear Orange
Nuclear Orange (Ex-Max 514 nm/Em-Max 554 nm)
Nuclear Orange from AAT Bioquest is a cell-permeant, fluorogenic DNA binding dye that is excited at 514 nm and emits at 556 nm. It is used in the following applications: Flow Cytometry, Fluorescence Microscopy.
Excitation & emission spectra
Key facts
- Excitation maximum
- 514 nm
- Emission maximum
- 554 nm
- Optimal laser line
- 488
- Optimal filter
- 575/36
Nuclear Orange has an excitation maximum at 514 nm and an emission maximum at 554 nm, a Stokes shift of 40 nm. It is optimally excited by the 488 laser line.
Frequently asked questions
- What is Nuclear Orange?
- Nuclear Orange from AAT Bioquest is a cell-permeant, fluorogenic DNA binding dye that is excited at 514 nm and emits at 556 nm. It is used in the following applications: Flow Cytometry, Fluorescence Microscopy.
- What laser excites Nuclear Orange?
- Nuclear Orange is optimally excited by the 488 laser line. Its excitation maximum is 514 nm.
- What filter should I use to detect Nuclear Orange?
- A 575/36 filter captures the peak of the Nuclear Orange emission near 554 nm with minimal spillover.