MitoLite Red FX600
MitoLite Red FX600 (Ex-Max 579 nm/Em-Max 600 nm)
MitoLite Red FX600 from AAT Bioquest is a potential-dependent red fluorescent dye used to stain mitochondria in live cells. It has an excitation max at 579 nm and an emission peak at 600 nm. It is recommened for use in fluorescence microscopy and flow cytometry, and is retained with paraformaldehyde fixation.
Excitation & emission spectra
Key facts
- Excitation maximum
- 579 nm
- Emission maximum
- 600 nm
- Optimal laser line
- 561
- Optimal filter
- 610/20
MitoLite Red FX600 has an excitation maximum at 579 nm and an emission maximum at 600 nm, a Stokes shift of 21 nm. It is optimally excited by the 561 laser line.
Frequently asked questions
- What is MitoLite Red FX600?
- MitoLite Red FX600 from AAT Bioquest is a potential-dependent red fluorescent dye used to stain mitochondria in live cells. It has an excitation max at 579 nm and an emission peak at 600 nm. It is recommened for use in fluorescence microscopy and flow cytometry, and is retained with paraformaldehyde fixation.
- What laser excites MitoLite Red FX600?
- MitoLite Red FX600 is optimally excited by the 561 laser line. Its excitation maximum is 579 nm.
- What filter should I use to detect MitoLite Red FX600?
- A 610/20 filter captures the peak of the MitoLite Red FX600 emission near 600 nm with minimal spillover.