MitoLite Orange FX570
MitoLite Orange FX570 (Ex-Max 551 nm/Em-Max 570 nm)
MitoLite Orange FX570 from AAT Bioquest is a potential-dependent orange fluorescent dye used to stain mitochondria in live cells. It has an excitation max at 551 nm and an emission peak at 570 nm. It is recommened for use in fluorescence microscopy and flow cytometry, and is retained with paraformaldehyde fixation.
Excitation & emission spectra
Key facts
- Excitation maximum
- 551 nm
- Emission maximum
- 570 nm
- Optimal laser line
- 561
- Optimal filter
- 585/42
MitoLite Orange FX570 has an excitation maximum at 551 nm and an emission maximum at 570 nm, a Stokes shift of 19 nm. It is optimally excited by the 561 laser line.
Frequently asked questions
- What is MitoLite Orange FX570?
- MitoLite Orange FX570 from AAT Bioquest is a potential-dependent orange fluorescent dye used to stain mitochondria in live cells. It has an excitation max at 551 nm and an emission peak at 570 nm. It is recommened for use in fluorescence microscopy and flow cytometry, and is retained with paraformaldehyde fixation.
- What laser excites MitoLite Orange FX570?
- MitoLite Orange FX570 is optimally excited by the 561 laser line. Its excitation maximum is 551 nm.
- What filter should I use to detect MitoLite Orange FX570?
- A 585/42 filter captures the peak of the MitoLite Orange FX570 emission near 570 nm with minimal spillover.