MitoLite Orange EX405
MitoLite Orange EX405 (Ex-Max 425 nm/Em-Max 519 nm)
MitoLite Orange EX405 from AAT Bioquest is a potential-dependent orange fluorescent dye used to stain mitochondria in live cells. It has an excitation max at 399 nm and an emission peak at 550 nm. It is recommened for use in fluorescence microscopy and flow cytometry, but is not retained with paraformaldehyde fixation.
Excitation & emission spectra
Key facts
- Excitation maximum
- 399 nm
- Emission maximum
- 550 nm
- Optimal laser line
- 405
- Optimal filter
- 515/30
MitoLite Orange EX405 has an excitation maximum at 399 nm and an emission maximum at 550 nm, a Stokes shift of 151 nm. It is optimally excited by the 405 laser line.
Frequently asked questions
- What is MitoLite Orange EX405?
- MitoLite Orange EX405 from AAT Bioquest is a potential-dependent orange fluorescent dye used to stain mitochondria in live cells. It has an excitation max at 399 nm and an emission peak at 550 nm. It is recommened for use in fluorescence microscopy and flow cytometry, but is not retained with paraformaldehyde fixation.
- What laser excites MitoLite Orange EX405?
- MitoLite Orange EX405 is optimally excited by the 405 laser line. Its excitation maximum is 399 nm.
- What filter should I use to detect MitoLite Orange EX405?
- A 515/30 filter captures the peak of the MitoLite Orange EX405 emission near 550 nm with minimal spillover.