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MitoLite NIR FX690

MitoLite NIR FX690 (Ex-Max 660 nm/Em-Max 690 nm)

MitoLite NIR FX690 from AAT Bioquest is a potential-dependent red fluorescent dye used to stain mitochondria in live cells. It has an excitation max at 660 nm and an emission peak at 690 nm. It is recommened for use in fluorescence microscopy and flow cytometry, and is retained with paraformaldehyde fixation.

Excitation & emission spectra

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Key facts

Excitation maximum
660 nm
Emission maximum
690 nm
Optimal laser line
628-640
Optimal filter
695/40

MitoLite NIR FX690 has an excitation maximum at 660 nm and an emission maximum at 690 nm, a Stokes shift of 30 nm. It is optimally excited by the 628-640 laser line.

Frequently asked questions

What is MitoLite NIR FX690?
MitoLite NIR FX690 from AAT Bioquest is a potential-dependent red fluorescent dye used to stain mitochondria in live cells. It has an excitation max at 660 nm and an emission peak at 690 nm. It is recommened for use in fluorescence microscopy and flow cytometry, and is retained with paraformaldehyde fixation.
What laser excites MitoLite NIR FX690?
MitoLite NIR FX690 is optimally excited by the 628-640 laser line. Its excitation maximum is 660 nm.
What filter should I use to detect MitoLite NIR FX690?
A 695/40 filter captures the peak of the MitoLite NIR FX690 emission near 690 nm with minimal spillover.