MitoLite Green EX488
MitoLite Green EX488 (Ex-Max 509 nm/Em-Max 525 nm)
MitoLite Green EX488 AAT from Bioquest is a potential-dependent green fluorescent dye used to stain mitochondria in live cells. It has an excitation max at 509 nm and an emission peak at 525 nm. It is recommened for use in fluorescence microscopy and flow cytometry, and is not retained with paraformaldehyde fixation.
Excitation & emission spectra
Key facts
- Excitation maximum
- 509 nm
- Emission maximum
- 525 nm
- Optimal laser line
- 488
- Optimal filter
- 515/30
MitoLite Green EX488 has an excitation maximum at 509 nm and an emission maximum at 525 nm, a Stokes shift of 16 nm. It is optimally excited by the 488 laser line.
Frequently asked questions
- What is MitoLite Green EX488?
- MitoLite Green EX488 AAT from Bioquest is a potential-dependent green fluorescent dye used to stain mitochondria in live cells. It has an excitation max at 509 nm and an emission peak at 525 nm. It is recommened for use in fluorescence microscopy and flow cytometry, and is not retained with paraformaldehyde fixation.
- What laser excites MitoLite Green EX488?
- MitoLite Green EX488 is optimally excited by the 488 laser line. Its excitation maximum is 509 nm.
- What filter should I use to detect MitoLite Green EX488?
- A 515/30 filter captures the peak of the MitoLite Green EX488 emission near 525 nm with minimal spillover.