Calcein Red
Calcein Red (Ex-Max 561 nm/Em-Max 571 nm)
Calcein Red is the hydrodlyzed version of Calcein Red, AM. It is commonly used as a cell impermeant, esterase independet control for Calcein Red, AM. Commonly used in fluorescence microscopy, flow cytometry, or fluorescent microplate reader assays. It has an excitation peak at 561 nm and an emission peak at 571 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 561 nm
- Emission maximum
- 571 nm
- Optimal laser line
- 561
- Optimal filter
- 585/42
Calcein Red has an excitation maximum at 561 nm and an emission maximum at 571 nm, a Stokes shift of 10 nm. It is optimally excited by the 561 laser line.
Frequently asked questions
- What is Calcein Red?
- Calcein Red is the hydrodlyzed version of Calcein Red, AM. It is commonly used as a cell impermeant, esterase independet control for Calcein Red, AM. Commonly used in fluorescence microscopy, flow cytometry, or fluorescent microplate reader assays. It has an excitation peak at 561 nm and an emission peak at 571 nm.
- What laser excites Calcein Red?
- Calcein Red is optimally excited by the 561 laser line. Its excitation maximum is 561 nm.
- What filter should I use to detect Calcein Red?
- A 585/42 filter captures the peak of the Calcein Red emission near 571 nm with minimal spillover.