Calcein Deep Red
Calcein Deep Red (Ex-Max 645 nm/Em-Max 655 nm)
Calcein Deep Red is the hydrodlyzed version of Calcein Deep Red, AM. It is commonly used as a cell impermeant, esterase independet control for Calcein Deep Red, AM. Commonly used in fluorescence microscopy, flow cytometry, or fluorescent microplate reader assays. It has an excitation peak at 645 nm and an emission peak at 655 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 645 nm
- Emission maximum
- 655 nm
- Optimal laser line
- 628-640
- Optimal filter
- 660/20
- Molecular weight
- 373.66
Calcein Deep Red has an excitation maximum at 645 nm and an emission maximum at 655 nm, a Stokes shift of 10 nm. It is optimally excited by the 628-640 laser line.
Frequently asked questions
- What is Calcein Deep Red?
- Calcein Deep Red is the hydrodlyzed version of Calcein Deep Red, AM. It is commonly used as a cell impermeant, esterase independet control for Calcein Deep Red, AM. Commonly used in fluorescence microscopy, flow cytometry, or fluorescent microplate reader assays. It has an excitation peak at 645 nm and an emission peak at 655 nm.
- What laser excites Calcein Deep Red?
- Calcein Deep Red is optimally excited by the 628-640 laser line. Its excitation maximum is 645 nm.
- What filter should I use to detect Calcein Deep Red?
- A 660/20 filter captures the peak of the Calcein Deep Red emission near 655 nm with minimal spillover.