MitoLite Blue FX490
MitoLite Blue FX490 (Ex-Max 350 nm/Em-Max 467 nm)
MitoLite Blue FX490 from AAT Bioquest is a potential-dependent blue fluorescent dye used to stain mitochondria in live cells. It has an excitation max at 344 nm and an emission peak at 469 nm. It is recommened for use in fluorescence microscopy and flow cytometry, and is retained with paraformaldehyde fixation.
Excitation & emission spectra
Key facts
- Excitation maximum
- 344 nm
- Emission maximum
- 469 nm
- Optimal laser line
- 355
- Optimal filter
- 450/50
MitoLite Blue FX490 has an excitation maximum at 344 nm and an emission maximum at 469 nm, a Stokes shift of 125 nm. It is optimally excited by the 355 laser line.
Frequently asked questions
- What is MitoLite Blue FX490?
- MitoLite Blue FX490 from AAT Bioquest is a potential-dependent blue fluorescent dye used to stain mitochondria in live cells. It has an excitation max at 344 nm and an emission peak at 469 nm. It is recommened for use in fluorescence microscopy and flow cytometry, and is retained with paraformaldehyde fixation.
- What laser excites MitoLite Blue FX490?
- MitoLite Blue FX490 is optimally excited by the 355 laser line. Its excitation maximum is 344 nm.
- What filter should I use to detect MitoLite Blue FX490?
- A 450/50 filter captures the peak of the MitoLite Blue FX490 emission near 469 nm with minimal spillover.