LysoBrite Orange
LysoBrite Orange (Ex-Max 540 nm/Em-Max 555 nm)
LysoBrite Orange from AAT Bioquest is a cell-permeant alkaline probe that localizes to acidic compartments inside the cell, such as endosomes and lysosomes. These types of probes are designed for live-cell imaging and do not typically survive fixation and permeabilization. LysoBrite Orange has an excitation peak at 540 nm and an emission peak at 555 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 540 nm
- Emission maximum
- 555 nm
- Optimal laser line
- 532
- Optimal filter
- 550/40
LysoBrite Orange has an excitation maximum at 540 nm and an emission maximum at 555 nm, a Stokes shift of 15 nm. It is optimally excited by the 532 laser line.
Frequently asked questions
- What is LysoBrite Orange?
- LysoBrite Orange from AAT Bioquest is a cell-permeant alkaline probe that localizes to acidic compartments inside the cell, such as endosomes and lysosomes. These types of probes are designed for live-cell imaging and do not typically survive fixation and permeabilization. LysoBrite Orange has an excitation peak at 540 nm and an emission peak at 555 nm.
- What laser excites LysoBrite Orange?
- LysoBrite Orange is optimally excited by the 532 laser line. Its excitation maximum is 540 nm.
- What filter should I use to detect LysoBrite Orange?
- A 550/40 filter captures the peak of the LysoBrite Orange emission near 555 nm with minimal spillover.