BODIPY 558/568
BODIPY 558/568 (Ex-Max 555 nm/Em-Max 566 nm)
BODIPY 558/568 is used as a lipophilic probe for polar and neutral lipids typically used to detect oil and nonpolar lipid droplets in live or fixed cells. It has an excitation peak at 558 nm and an emission peak at 568 nm. The succinimidyl ester form of this dye can be used to conjugate hydrophobic proteins or lipids.
Excitation & emission spectra
Key facts
- Excitation maximum
- 555 nm
- Emission maximum
- 566 nm
- Optimal laser line
- 561
- Optimal filter
- 575/36
- Molecular weight
- 472.3989
BODIPY 558/568 has an excitation maximum at 555 nm and an emission maximum at 566 nm, a Stokes shift of 11 nm. It is optimally excited by the 561 laser line.
Frequently asked questions
- What is BODIPY 558/568?
- BODIPY 558/568 is used as a lipophilic probe for polar and neutral lipids typically used to detect oil and nonpolar lipid droplets in live or fixed cells. It has an excitation peak at 558 nm and an emission peak at 568 nm. The succinimidyl ester form of this dye can be used to conjugate hydrophobic proteins or lipids.
- What laser excites BODIPY 558/568?
- BODIPY 558/568 is optimally excited by the 561 laser line. Its excitation maximum is 555 nm.
- What filter should I use to detect BODIPY 558/568?
- A 575/36 filter captures the peak of the BODIPY 558/568 emission near 566 nm with minimal spillover.